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Oggetto:
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In vitro and ex vivo approaches to study rodent and human neural cells

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Disciplinary Training (Basic Neuroscience)

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Anno accademico 2021/2022

Docenti
Prof.ssa Annalisa Buffo (Titolare del corso)
Prof.ssa Enrica Boda (Titolare del corso)
Corso di studio
[502204] NEUROSCIENZE
Anno
1° anno
Periodo
Secondo semestre
Tipologia
indirizzo Neuroscienze Sperimentali
Crediti/Valenza
2,5
SSD attività didattica
BIO/09 - fisiologia
BIO/16 - anatomia umana
Erogazione
Mista
Lingua
Inglese
Frequenza
Obbligatoria
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Sommario insegnamento

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Obiettivi formativi

Specific objectives and learning outcomes:

The course will provide an overview in vitro and ex vivo approaches/models that are currently used to investigate the cellular and molecular mechanisms underlying  specific  aspects  of  neural  (neuronal  and  glial )  cell  development, functioning,  crosstalk  and  pathology.  This will include recent advances in human pluripotent stem cells (hPSC) differentiation, based on the timely manipulation of signaling pathways known  to  govern  development  of  specific  tissues  in embryos,  to  generate  pertinent  cellular  models.  The course will also expand on state of the art approaches to unveil heterogeneity in cell states or cell types.

Students will gain knowledge useful to identify the best reductionistic approach to answer their research questions.

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Risultati dell'apprendimento attesi

After completion of the course, the student will understand how in vitro/ex vivo systems can be used to investigate specific aspects of neural cell physiopathology, and their advantages and limitations compared to in vivo models.

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Programma

Course content:

Establishment, applications, advantages and limitations of:

-  Primary neuronal and glial cell cultures (isolated cultures and co-culture methods)

-  Neural cell lines

-  CNS-derived organotypic cultures

-  Methods for isolating specific cell populations from the embryonic/postnatal and adult CNS (MACS- magnetic-activated cell sorting)

-  Flow cytometric analysis and isolation (FACS): principles and data interpretation

-  hPSC derived neurons and glia

-  hPSC derived 2D and 3D models for tissue and disease modelling

-  Multiplex approaches for spatial gene expression profiling at single-cell and subcellular resolution.

The course will include a theoretical part as well as the design by the attendees of an experiment based on one or more of methodologies/approaches among those discussed. The experiment proposal will be presented and discussed together on July 4.

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Modalità di insegnamento

Dates:

June 28 (h 10-13; h 14-17)

July 4 (h 14-18)

Format: Lecture-Presentation-Discussion

Course delivery:

Blended – in presence classes at NICO, Regione Gonzole 10 Orbassano Torino

 

 

 

 

Testi consigliati e bibliografia

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Please see uploads on Moodle



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Note

Course director/ teachers

Name/s  Annalisa Buffo, Enrica Boda

e-mail:  annalisa.buffo@unito.it enrica.boda@unito.it

 

Other teachers:

Valentina Cerrato, UNITO valentina.cerrato@unito.it

Gianmarco Pallavicini, UNITO gianmarco.pallavicini@unito.it

Elena Parmigiani, University of Basel elena.parmigiani@unibas.ch

Registrazione
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    Ultimo aggiornamento: 30/05/2022 12:53